Pueraria lobata extract
This product is extracted from dried root of Pueraria lobata WILLD. OHWI, Pueraria pseudo-hirsuta TANG et WANG, Pueraria thomsonii BENTN by water and ethanol.
Active ingredients: daidzein, daidzin, puerarin, puerarin-7-xyloside,
Specification:
Pueraria flavonoid:10%~80% by UV
Puerarin:10%~98% by HPLC
Appearance: Brown to yellow powder or granular
Test Standard: CP2020
Composition: 100% natural extract , Non-irradiation, Non-GMO
Other test details , please email us for COA.
Packing:25KG/drum or 1KG/bag
Shelf time: 2year
In vitro study, puerarin inhibited the expression of iNOS,COX-2 and CRP proteins induced by LPS, and also inhibited their mRNA in RT-PCR assay in RAW264.7 cells. The inhibition of iNOS,COX-2, and CRP expression is due to dose-dependent inhibition of phosphorylation and degradation of I-κB, resulting in a reduction in nuclear translocation of p65NF-κB. Puerarin mediated inhibition of LPS-induced iNOS,COX-2, and CRP expression is attributed to inhibition of NF-κB activation at the transcriptional level [1]. Puerarin is a novel IK1 open channel blocker, which may be the basis of the antiarrhythmic effect of puerarin. Puerarin competes with barium, an open channel blocker of IK1, to inhibit IK1 current [2].
In vivo studies, both genistein and puerarin can effectively alleviate chronic alcohol-induced liver damage through potential antioxidant, anti-inflammatory, or anti-apoptotic mechanisms. However, genioflavin was more effective than puerarin in reducing malondialdehyde levels (1.05±0.0947 vs. 1.28±0.213 nmol/mg pro,p <0.05), tumor necrosis factor α(3.12±0.498 vs. 3.82±0.277 pg/mg pro, P <0.05), and tumor necrosis factor α(3.12±0.498 vs. 3.82±0.277 pg/mg Pro, P <0.05). p <0.05), interleukin-6 (1.46±0.223 vs. 1.88±0.309 pg/mg pro,p <0.05), while puerarin was more effective than tigoflavin in improving serum activity or alanine aminotransferase levels (35.8±3.95 vs. 42.6) ±6.56 U/L,p <0.05) and low density lipoprotein cholesterol (1.12±0.160 vs. 1.55±0.150 mmol/L,p <0.05)[3]. Puerarin can significantly ameliorate early renal damage, possibly by inhibiting the expression of ICAM-1 and TNF-α in the kidneys of diabetic rats [4].
RAW264.7 cells were kept in a subconfluent state at 37ºC in 95% air and 5% CO2 humid atmosphere. The medium used for routine passage culture is Dulbecco's Modified Eagle's medium supplemented with 10% fetal bovine serum, penicillin (100 units/mL), and streptomycin (100μg/ mL). The MTT assay was used to measure the viability of cells treated with puerarin. After the supernatant was removed for nitrite determination, the cells were incubated at 37ºC with MTT (0.05mg/mL) for 4 hours, and the optical density was measured at 540nm. The concentrations of puerarin were 10,20,40 and 100μM, respectively [1].
Animal experimental rats: A group of healthy male SD rats (7 weeks of age) were randomly divided into a control group, a model group, and a puerarin treatment group with high (H), moderate (M), and low (L) doses. Puerarin was re-suspended in 0.9% normal saline and administered by gastric cannula of different concentrations (0.25 mg/(kg×d) in group L, 0.5 mg/(kg×d) in group M, 1.0 mg/() per kg×d per day for 8 consecutive days). Equal amounts of saline were given over the same time period to control and simulate the rats [4]. Mice: 40 male ICR mice (weight: 20-22g) were adapted to 12 hours of daily light: 12 hours of darkness cycle at 22±2ºC room temperature and 55% ± 5% relative humidity. After 1 week of adaptation, the mice were randomly divided into 4 groups with 10 mice in each group. Cephaloflavin and puerarin were administered to mice with sodium carboxymethyl cellulose solution at an equal molar concentration of 0.1M (stomach volume: 3mL kg-1 body weight) [3].
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